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Mode of Action (MoA)
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AlphaFold 3 predicts a direct interaction between Cas8a1 (https://www.ncbi.nlm.nih.gov/protein/WWV39459.1) and AcrIB4 (ipTM = 0.91, pTM = 0.76).
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Evidence
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Binding it supported by computational structure modeling evidence (ECO_0006368).Ectopic expression of AcrIB4 (ECO_0000017) abolished interference against target plasmid. Expression of AcrIB4 blocks CRISPR interference. AcrIB4 did not disrupt Cascade assembly (immunoprecipitation).
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MoA Category
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binds and inhibits host defence system (putative)
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Listeria seeligeri type I-B CRISPR-Cas
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Relevant publication(s)
DOI
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10.1038/s41586-024-07923-x
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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MGE and prophages in Listeria genomes
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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