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Mode of Action (MoA)
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None
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Evidence
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AcrIC11 was identified from Xanthomonas albilineans and tested for its ability to inhibit the Type I-C CRISPR-Cas system. In E. coli Cas3 degradation assays, co-expression of Cascade, targeting crRNA, and Cas3 led to cell death due to chromosomal targeting. Co-expression of AcrIC11 restored colony formation, indicating effective inhibition. In vitro TXTL assays confirmed that AcrIC11 inhibited DNA degradation but not DNA binding by Cascade. deGFP repression assays showed no significant impact on Cascade-DNA binding, while degradation assays revealed ~60% inhibition of Cas3 activity. AlphaFold-based structural comparison with KlcA supports functional homology (RMSD = 1.467 Å), consistent with nuclease-targeting activity.
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MoA Category
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unknown
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Xanthomonas albilineans CFBP7063 type I-C CRISPR-Cas system
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Relevant publication(s)
DOI
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10.1093/nar/gkad1097
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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MGE in Xanthomonas albilineans
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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