|
Mode of Action (MoA)
|
AlphaFold 3 predicts a direct interaction between Cas8c (https://www.ncbi.nlm.nih.gov/protein/UEM35121.1) and AcrIC5 (ipTM = 0.92, pTM = 0.85).
|
|
Evidence
|
The acrIC5 gene was cloned into DMS3m and tested on both PAO1IC and a P. aeruginosa strain expressing a heterologous Eggerthella lenta Type I-C system. In both hosts, the phage carrying AcrIC5 formed plaques at wild-type levels (EOP ≈ 1). CRISPRi assays confirmed DNA binding was inhibited. This demonstrates AcrIC5 has broad activity across divergent I-C systems, despite only moderate Cas protein sequence identity (~35–55%). Binding it supported by computational structure modeling evidence (ECO_0006368).
|
|
MoA Category
|
binds and inhibits host defence system (putative)
|
|
Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
|
Pseudomonas aeruginosa type I-C CRISPR-Cas system
|
|
Relevant publication(s)
DOI
|
10.1093/nar/gkab006
|
|
Other components of the anti-defence system
Multicomponent System
|
-
|
|
Known structure in PDB
PDB ID
|
-
|
|
Genome(s) encoding the protein
Protein Source
|
MGE in Pseudomonas delhiensis
|
|
Defence system(s) inhibited by the protein
Defences
|
CRISPR-Cas
|