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Mode of Action (MoA)
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inactivates Cascade by trapping the PAM-recognising Cas8 subunit in a non-productive conformation, incapable of performing PAM recognition.
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Evidence
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DMS3m phage engineered to express acrIC8 was tested on PAO1IC and a Type I-E strain (PA4386). The phage regained full infectivity on both (EOP ≈ 1), and CRISPRi assays confirmed inhibition of DNA binding. AcrIC8 is a broad-spectrum Acr that blocks both Type I-C and Type I-E systems.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa type I-C CRISPR-Cas system
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Relevant publication(s)
DOI
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10.1093/nar/gkab006
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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8g9s_A
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Genome(s) encoding the protein
Protein Source
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MGE in Pseudomonas aeruginosa
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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