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Mode of Action (MoA)
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binds as a dimer to the Cas10d.
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Evidence
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A mutant virus, SIRV2M, with a 4 kb deletion (ECO_0001038) including the acrID1 gene, lost the ability to infect Sulfolobus islandicus LAL14/1, which has active CRISPR–Cas systems. Reintroduction (using homologous recombination) of SIRV3 gp02 (acrID1) alone into SIRV2M restored infectivity, indicating gp02 is necessary and sufficient for overcoming CRISPR immunity. Pull-down assays (ECO_0006249) and gel filtration chromatography (ECO_0001049) demonstrated that AcrID1 binds directly to Cas10d, the large subunit of the I-D effector complex.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Sulfolobus islandicus LAL14/1 type I-D CRISPR-Cas system
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Relevant publication(s)
DOI
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10.1038/s41564-018-0120-z
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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6EXP
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Genome(s) encoding the protein
Protein Source
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Sulfolobus islandicus rudivirus 3 isolate SIRV3
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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