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Mode of Action (MoA)
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binds as a dimer to Cas3 (blocks DNA cleavage).
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Evidence
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AcrIE1 was identified via plaque assays in Pseudomonas aeruginosa strain SMC4386 expressing individual anti-CRISPR genes from plasmids. Expression (ECO_0000017) of ACR88a-32 from phage JBD88a allowed robust infection by CRISPR-sensitive phage JBD8, which otherwise fails to infect due to targeting by the type I-E CRISPR-Cas system. Transformation efficiency assays in lysogenized strains confirmed loss of CRISPR interference, supporting ACR88a-32’s inhibitory role.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa SMC4386 type I-E CRISPR-Cas
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Relevant publication(s)
DOI
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10.1128/mBio.00896-14
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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6AS4
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Genome(s) encoding the protein
Protein Source
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Pseudomonas phage JBD5
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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