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Mode of Action (MoA)
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primarily binds to Cas8e, but may interact with Cas5e and Cas7e.
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Evidence
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AcrIE3 activity was demonstrated by expressing ACR3112-31 from phage D3112 in P. aeruginosa SMC4386. The expression (ECO_0000017) enabled phage JBD8, which is normally blocked by the CRISPR system, to form plaques at a 10³–10⁵-fold increased efficiency compared to controls. Transformation assays in lysogenized strains supported functional suppression of type I-E activity.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa SMC4386 type I-E CRISPR-Cas
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Relevant publication(s)
DOI
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10.1128/mBio.00896-14,
10.1016/j.str.2024.10.024
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Pseudomonas phage DMS3
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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