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Mode of Action (MoA)
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AlphaFold 3 predicts high-confidence interactions between CasA (Cas8e, https://www.ncbi.nlm.nih.gov/protein/QZE32573.1) and AcrIE4 (ipTM = 0.92, pTM = 0.89) and Cas6e (https://www.ncbi.nlm.nih.gov/protein/QZE32569.1) and AcrIE4 (ipTM = 0.8, pTM = 0.85).
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Evidence
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AcrIE4 was identified in phage JBD5 and shown to inhibit the type I-E system using both transformation efficiency assays and plaque assays. Expression (ECO_0000017) of ACR5-34 from a plasmid in SMC4386 enabled efficient plaque formation by CRISPR-targeted phage JBD8. Additionally, a homolog of ACR5-34 found in a P. aeruginosa mobile genetic element (non-phage) was also shown to have anti-CRISPR activity in plaque assays, suggesting this gene may contribute to horizontal gene transfer by evading CRISPR immunity. Binding it supported by computational structure modeling evidence (ECO_0006368).
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MoA Category
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binds and inhibits host defence system (putative)
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa SMC4386 type I-E CRISPR-Cas
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Relevant publication(s)
DOI
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10.1128/mBio.00896-14
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Pseudomonas phage D3112
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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