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Mode of Action (MoA)
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its N-terminal domain targets the PAM interaction site of the Cas8e subunit, and the C-terminal domain disables target DNA recognition at the PAM interaction site in the Cas8f subunit.
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Evidence
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AcrIE4-IF7 is a chimeric protein that inhibits both type I-E and I-F CRISPR-Cas systems. Upon ectopic expression in P. aeruginosa (ECO_0000017), it restored replication of phages JBD8 (I-E) and DMS3m (I-F), confirming dual activity.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa SMC4386 type I-E and I-F CRISPR-Cas
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Relevant publication(s)
DOI
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10.1126/science.aau5174,
10.1093/nar/gkac096
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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7VZM
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Genome(s) encoding the protein
Protein Source
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MGE in Pseudomonas aeruginosa SMC4386
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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