|
Mode of Action (MoA)
|
AlphaFold 3 predicts high-confidence interactions between Cas5e (https://www.ncbi.nlm.nih.gov/protein/QZE32570.1) and AcrIE5 (ipTM = 0.81, pTM = 0.86).
|
|
Evidence
|
In Pseudomonas aeruginosa strains ectopically expressing AcrIE5 (ECO_0000017) and harboring active type I-E CRISPR-Cas targeting phage JBD8, phage replication was restored, demonstrating CRISPR inhibition. Binding it supported by computational structure modeling evidence (ECO_0006368).
|
|
MoA Category
|
binds and inhibits host defence system (putative)
|
|
Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
|
Pseudomonas aeruginosa SMC4386 type I-E CRISPR-Cas
|
|
Relevant publication(s)
DOI
|
10.1126/science.aau5174
|
|
Other components of the anti-defence system
Multicomponent System
|
-
|
|
Known structure in PDB
PDB ID
|
-
|
|
Genome(s) encoding the protein
Protein Source
|
MGE in Pseudomonas aeruginosa SMC4386
|
|
Defence system(s) inhibited by the protein
Defences
|
CRISPR-Cas
|