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Mode of Action (MoA)
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directly binds Cas7, sterically occluding target DNA and preventing its hybridisation to the crRNA.
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Evidence
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Phage JBD30 evades type I-F CRISPR-Cas immunity in P. aeruginosa PA14 through expression of AcrIF1 (gene 35). A frameshift mutation in gene 35 abolished infectivity on CRISPR-proficient hosts (ECO_0007269). Complementation with plasmid-expressed AcrIF1 restored phage replication (ECO_0000017).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa PA14 type I-F CRISPR-Cas
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Relevant publication(s)
DOI
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10.1038/nature11723
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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5uz9_I
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Genome(s) encoding the protein
Protein Source
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Pseudomonas phage JBD30
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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