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Mode of Action (MoA)
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AlphaFold 3 predicts a direct interaction between Cas8f/Csy1 (AHH51210.1) and AcrIF18* (ipTM = 0.71, pTM = 0.61).
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Evidence
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Ectopic expression (ECO_0000017) of AcrIF18 increased the plating efficiency of phages that are normally targeted by active type I-F CRISPR–Cas systems. Prevents CRISPRi, indicating inhibition occurs upstream of DNA binding. Binding it supported by computational structure modeling evidence (ECO_0006368).
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MoA Category
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binds and inhibits host defence system (putative)
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa type I-F and I-E CRISPR-Cas
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Relevant publication(s)
DOI
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10.1038/s41467-020-19415-3
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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MGE in Serratia marcescens
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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