|
Mode of Action (MoA)
|
binds to the Cas3 nuclease and locks it in the ADP-bound form, thus preventing it from binding to the effector-DNA complex and cleaving the target DNA.
|
|
Evidence
|
Phage MP29 expresses AcrF3 (gene 29), which blocks CRISPR interference in P. aeruginosa PA14. Plasmid-based expression of AcrF3 restored transformation of targeted plasmids and increased phage plaquing efficiency (ECO_0000016, ECO_0000017).
|
|
MoA Category
|
binds and inhibits host defence system
|
|
Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
|
Pseudomonas aeruginosa PA14 type I-F CRISPR-Cas
|
|
Relevant publication(s)
DOI
|
10.1038/nature11723
|
|
Other components of the anti-defence system
Multicomponent System
|
-
|
|
Known structure in PDB
PDB ID
|
5GQH_B
|
|
Genome(s) encoding the protein
Protein Source
|
Pseudomonas phage JBD5
|
|
Defence system(s) inhibited by the protein
Defences
|
CRISPR-Cas
|