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Mode of Action (MoA)
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binds to Cascade, the RNA-guided Csy complex, blocking DNA binding.
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Evidence
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AcrF4 (gene 30) was cloned from phage D3112 and shown to counteract CRISPR immunity in P. aeruginosa. Plasmid expression of AcrF4 in PA14 enhanced replication of CRISPR-sensitive phages (ECO_0000017).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa PA14 type I-F CRISPR-Cas
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Relevant publication(s)
DOI
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10.1038/nature11723
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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7YHS_J
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Genome(s) encoding the protein
Protein Source
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Pseudomonas phage JBD24
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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