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Mode of Action (MoA)
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binds to the Csy–dsDNA complex, destabilising the helical bundle domain of Cas8f and thus preventing subsequent Cas2/3 recruitment.
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Evidence
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The AcrF5 protein, encoded by gene 33 of phage JBD88a, enables escape from CRISPR interference in type I-F systems. Expression in P. aeruginosa PA14 permitted replication of targeted phages and efficient transformation of CRISPR-targeted plasmids (ECO_0000017).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa PA14 type I-F CRISPR-Cas
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Relevant publication(s)
DOI
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10.1038/nature11723
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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7F45
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Genome(s) encoding the protein
Protein Source
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Pseudomonas phage JBD5
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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