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Mode of Action (MoA)
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binds to the target DNA-binding site of Cas8f.
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Evidence
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Ectopic expression of AcrF7 in Pseudomonas aeruginosa with a type I-F CRISPR-Cas system lead to restoration of phage replication with CRISPR-sensitive phage. AcrIF7 inhibited CRISPR interference. Electrophoretic Mobility Shift Assays (ECO_0000096) shows binding to the Csy complex. Pull-down experiments and gel filtration, indicating specific interaction with Cas8f (ECO_0006249).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa type I-F CRISPR-Cas
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Relevant publication(s)
DOI
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10.1038/nmicrobiol.2016.85,
10.1093/nar/gkaa690
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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6M3N
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Genome(s) encoding the protein
Protein Source
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Pseudomonas phage LPB1
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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