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Mode of Action (MoA)
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binds to the Csy spiral backbone, occupying the cavity surrounded by Cas5f, Cas7.4–7.6f, and Cas8f, to prevent DNA hybridisation.
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Evidence
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Ectopic expression (ECO_0000017) of AcrIF8 in Pseudomonas aeruginosa resulted in inhibition of type I-F CRISPR-Cas system activity, as demonstrated by restored replication of CRISPR-targeted bacteriophages in plaque assays.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa type I-F CRISPR-Cas
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Relevant publication(s)
DOI
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10.1038/nmicrobiol.2016.85
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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6VQW_A
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Genome(s) encoding the protein
Protein Source
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Pectobacterium phage ZF40
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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