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Mode of Action (MoA)
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binds Cas9, triggering its degradation during lysogeny.
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Evidence
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Genetic deletions of acrIIA1 (deletion mutation phenotypic evidence, ECO_0001038) in the prophage result in restored CRISPR-Cas9 function, as demonstrated by regained plasmid-targeting ability in Listeria monocytogenes. Conversely, ectopic expression of gene (ECO_0000017) in a CRISPR-active, prophage-cured background inhibits CRISPR-Cas9 activity, restoring transformation/plating efficiency with a targeted plasmid. Binding is supported by x-ray crystallography evidence (ECO_0005670).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Listeria monocytogenes type II-A and type II-C CRISPR-Cas
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Relevant publication(s)
DOI
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10.1016/j.cell.2016.12.009,
10.1093/nar/gkx1181
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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5Y6A
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Genome(s) encoding the protein
Protein Source
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Listeria monocytogenes prophage 10403S
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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