|
Mode of Action (MoA)
|
AlphaFold 3 predicts a direct interaction between SauCas9 and AcrIIA13 (ipTM = 0.9, pTM = 0.79), which is supported by a PDB entry without a corresponding publication (https://www.rcsb.org/structure/7ENI).
|
|
Evidence
|
AcrIIA13 was cloned into a plasmid and tested in a TXTL assay, where it restored GFP expression otherwise suppressed by SauCas9 (ECO_0000017), confirming inhibition. In vitro cleavage assays showed that AcrIIA13 blocked SauCas9-mediated DNA cleavage, primarily by preventing DNA binding. RNA EMSA confirmed it did not interfere with RNP formation (ECO_0001807). Binding it supported by computational structure modeling evidence (ECO_0006368).
|
|
MoA Category
|
binds and inhibits host defence system
|
|
Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
|
Staphylococcus aureus type II-A CRISPR-Cas
|
|
Relevant publication(s)
DOI
|
10.1073/pnas.1917668117
|
|
Other components of the anti-defence system
Multicomponent System
|
-
|
|
Known structure in PDB
PDB ID
|
8K4M
|
|
Genome(s) encoding the protein
Protein Source
|
MGE in Staphylococcus schleiferi
|
|
Defence system(s) inhibited by the protein
Defences
|
CRISPR-Cas
|