|
Mode of Action (MoA)
|
None
|
|
Evidence
|
AcrIIA14 was discovered through guilt-by-association analysis using genes co-occurring with an aca-like gene near AcrIIA13. AcrIIA14 was cloned and tested in a TXTL assay, where it restored GFP expression suppressed by SauCas9, indicating anti-CRISPR activity (ECO_0000017). In vitro cleavage assays showed complete inhibition of SauCas9 activity, despite RNA and DNA EMSAs showing no interference with RNP formation or DNA binding (ECO_0001807). This suggests AcrIIA14 blocks cleavage post-DNA binding. Deletion of the N-terminal domain reduced but did not eliminate activity (ECO_0007379).
|
|
MoA Category
|
unknown
|
|
Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
|
Staphylococcus aureus type II-A CRISPR-Cas
|
|
Relevant publication(s)
DOI
|
10.1073/pnas.1917668117
|
|
Other components of the anti-defence system
Multicomponent System
|
-
|
|
Known structure in PDB
PDB ID
|
7ENM
|
|
Genome(s) encoding the protein
Protein Source
|
MGE in Staphylococcus simulans
|
|
Defence system(s) inhibited by the protein
Defences
|
CRISPR-Cas
|