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Mode of Action (MoA)
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cleaves the single guide RNA (sgRNA), disrupting the ability of Cas9 to target DNA.
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Evidence
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Ectopic expression (ECO_0000017) of AcrIIA18 in a P. aeruginosa strain with a heterologous SpyCas9 CRISPR system restores replication of sensitive phage. AcrIIA16's expression inhibit CRISPR Interference, suggesting inhibiting of SpyCas9 at the step of target DNA binding or at an upstream stage. Co-immunoprecipitation (ECO_0005644) showed binding to Cas9.
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MoA Category
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degrades or sequesters molecules utilised by host defence systems
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Streptococcus pyogenes type II-A CRISPR-Cas
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Relevant publication(s)
DOI
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10.1038/s41564-020-0692-2
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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7VLM
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Genome(s) encoding the protein
Protein Source
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MGE in Streptococcus macedonicus
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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