|
Mode of Action (MoA)
|
None
|
|
Evidence
|
In vitro DNA cleavage assays with SpyCas9, SinCas9, SauCas9, and AsCas12a showed Strong inhibition of SinCas9, weak inhibition of SpyCas9 and no inhibition of SauCas9 or AsCas12a. Competition binding assay with AcrIIA2 shows ML1 binds Cas9–sgRNA and blocks AcrIIA2 binding.
|
|
MoA Category
|
unknown
|
|
Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
|
Streptococcus iniae type II-A CRISPR-Cas
|
|
Relevant publication(s)
DOI
|
10.1093/nar/gkaa219
|
|
Other components of the anti-defence system
Multicomponent System
|
-
|
|
Known structure in PDB
PDB ID
|
-
|
|
Genome(s) encoding the protein
Protein Source
|
MGE in Streptococcus iniae
|
|
Defence system(s) inhibited by the protein
Defences
|
CRISPR-Cas
|