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Mode of Action (MoA)
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binds to SpyCas9 via the REC3 domain, inhibiting DNA loading.
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Evidence
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AcrIIA29 was identified from a Streptococcus prophage and tested using a plasmid interference assay in E. coli. In this setup, E. coli cells were engineered to express St3Cas9 and an sgRNA targeting a co-transformed antibiotic resistance plasmid. Cas9-mediated cleavage of this plasmid reduced colony formation on selective media. Co-expression of AcrIIA29 partially restored colony formation, indicating moderate anti-CRISPR activity. In vitro cleavage assays revealed that AcrIIA29 inhibited DNA cleavage without affecting Cas9’s DNA binding ability.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Streptococcus thermophilus and Streptococcus pyogenes type II-A CRISPR-Cas
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Relevant publication(s)
DOI
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10.1093/nar/gkac099
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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MGE in Streptococcus pyogenes NS3335
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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