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Mode of Action (MoA)
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AlphaFold 3 predicts a direct interaction between Cas2 (https://www.ncbi.nlm.nih.gov/protein/ALU77912.1) and AcrIIA3 (ipTM = 0.7, pTM = 0.72).
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Evidence
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Ectopic expression (ECO_0000017) of acrIIA3 in L. monocytogenes inhibits CRISPR-Cas9 function in a plasmid targeting assay. Binding it supported by computational structure modeling evidence (ECO_0006368).
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MoA Category
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binds and inhibits host defence system (putative)
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Listeria monocytogenes type II-A CRISPR-Cas
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Relevant publication(s)
DOI
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10.1016/j.cell.2016.12.009
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Listeria monocytogenes prophage ΦSLCC2482
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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