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Mode of Action (MoA)
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None
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Evidence
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AcrIIA30 was identified in a Streptococcus genomic element and shown to be a specific inhibitor of St1Cas9. In the plasmid interference assay, E. coli cells expressing St1Cas9 and a plasmid-targeting sgRNA lost the targeted plasmid and failed to grow on selective media. Co-expression of AcrIIA30 restored growth, indicating effective inhibition. In vitro cleavage assays confirmed that AcrIIA30 blocked Cas9–DNA complex formation. EMSA showed that AcrIIA30 not only prevented DNA binding but also caused a supershift (ECO_0001807), suggesting potential dimerization or complex stabilization.
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MoA Category
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unknown
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Streptococcus thermophilus type II-A CRISPR-Cas
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Relevant publication(s)
DOI
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10.1093/nar/gkac099
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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MGE in Streptococcus gordonii NCTC7870
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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