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Mode of Action (MoA)
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None
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Evidence
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AcrIIA33 was discovered from Streptococcus DNA and tested using a system in E. coli designed to identify anti-CRISPR proteins. In this system, Cas9 cuts a plasmid that carries a red fluorescent gene called mCherry. Cutting this plasmid kills the bacteria because the plasmid is needed for survival. When AcrIIA33 was expressed, bacteria survived, meaning Cas9 was blocked. This showed that AcrIIA33 stops Cas9 from cutting DNA.
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MoA Category
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unknown
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Streptococcus pyogenes type II-A CRISPR-Cas
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Relevant publication(s)
DOI
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10.1093/nar/gkad995
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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MGE in Streptococcus equi DSM 20561
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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