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Mode of Action (MoA)
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inhibits Cas9 enzymatic activity and DNA unwinding, and blocks the movement of HNH, thereby preventing DNA binding.
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Evidence
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Ectopic expression (ECO_0000017) of acrIIA4 in L. monocytogenes, E. coli, and HEK293T cells effectively inhibits CRISPR-Cas9 and dCas9 activity. Binding is supported by x-ray crystallography evidence (ECO_0005670).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Listeria monocytogenes type II-A CRISPR-Cas
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Relevant publication(s)
DOI
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10.1016/j.cell.2016.12.009,
10.1038/nature22377
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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5XN4
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Genome(s) encoding the protein
Protein Source
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Listeria monocytogenes prophage ΦJ0161b
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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