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Mode of Action (MoA)
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Directly interacts with the Cmr-a complex
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Evidence
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Genetic deletion of acrIIIB1 (deletion mutation phenotypic evidence, ECO_0001038) from the SIRV2 viral genome results in loss of viral infectivity in Sulfolobus islandicus strains with subtype III-B CRISPR-Cas systems, demonstrating restored CRISPR activity. Conversely, ectopic expression of acrIIIB1 (ECO_0000017) in a CRISPR-active host rescues viral infectivity, indicating inhibition of CRISPR function. Binding to the Cmr-a effector complexes is supported by co-purification in pull-down assays (co-purification evidence, ECO_0006074).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Sulfolobus islandicus LAL14/1 type III-B CRISPR-Cas
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Relevant publication(s)
DOI
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10.1016/j.cell.2019.09.003
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Sulfolobus islandicus rod-shaped virus 2
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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