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Mode of Action (MoA)
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blocks the dissociation of cleaved target RNA from Cmr-α, inhibiting Cmr-α turnover and thereby suppressing Cas10 activities.
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Evidence
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The gene gp40 from SIRV3 (AcrIIIB2) was used to replace the known Acr gene (acrIIIB1) in SIRV2. The recombinant virus SIRV2ΔacrIIIB1+gp40 was able to infect S. islandicus LAL14/1, which has a functional type III-B CRISPR-Cas system. This restored infectivity demonstrated that gp40 compensates for the loss of acrIIIB1, confirming its role as an anti-CRISPR protein. Pull-down assays with HA-tagged AcrIIIB2 revealed direct interaction with the Cmr-a complex, a type III-B effector (ECO_0006074).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Sulfolobus islandicus type III-B CRISPR-Cas
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Relevant publication(s)
DOI
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10.1016/j.chom.2023.10.003
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Sulfolobus islandicus rod-shaped virus 3 isolate SIRV3
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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