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Mode of Action (MoA)
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inhibits type VI-A CRISPR immunity by causing crRNA degradation (may degrade crRNAs directly, but there is a possibility that AcrVIA2 prevents loading of crRNAs into Cas13).
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Evidence
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Ectopic expression of AcrVIA2 (ECO_0000017) abolished Cas13-dependent interference against a plasmid target in L. seeligeri, as demonstrated by plasmid-targeting assays. The same ectopic expression restored infection by a Cas13-sensitive phage (ϕLS59), indicating functional suppression of CRISPR immunity. A DEAD-box mutant (DEFD→AAFD) of AcrVIA2 (ECO_0000015) was expressed at similar levels but failed to inhibit Cas13 activity, confirming that the helicase motif is essential for function.
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MoA Category
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unknown
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Listeria seeligeri type VI-A CRISPR-Cas
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Relevant publication(s)
DOI
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10.1038/s41586-024-07923-x
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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MGE in Listeria seeligeri
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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View homologs from eukaryotic dsDNA viruses
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