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Mode of Action (MoA)
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binds to the Leptotrichia wadei Cas13a.
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Evidence
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Ectopic expression (ECO_0000017) of AcrVIA5 abolishes Cas13a interference in bacterial plaque and plasmid targeting assays. Purified AcrVIA5 strongly inhibits Cas13a cleavage in vitro (ECO_0000184). Co-immunoprecipitation confirms binding to Cas13a (ECO_0005644). Mutation studies show key residues required for inhibition (ECO_0001038).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Leptotrichia buccalis type VI-A CRISPR-Cas
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Relevant publication(s)
DOI
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10.1016/j.molcel.2020.03.033
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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MGE in Leptotrichia wadei
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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