|
Mode of Action (MoA)
|
None
|
|
Evidence
|
RB69 phages were serially passaged on E. coli cells containing DarTG1 to select for escape mutants, mutations in gene 61.2 (renamed adfA) were found in resistant phages. Ectopic expression of mutant gp61.2(adfA) or T4 homolog increased plaquing efficiency on DarTG1-expressing cells. ELTA assays showed inhibition of DarT1 toxin activity (no ADP-ribosylation of DNA) when RB69 expressed evolved adfA (ECO_0005801).
|
|
MoA Category
|
unknown
|
|
Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
|
Escherichia coli type IV TA system DarTG
|
|
Relevant publication(s)
DOI
|
10.1038/s41564-022-01153-5
|
|
Other components of the anti-defence system
Multicomponent System
|
-
|
|
Known structure in PDB
PDB ID
|
-
|
|
Genome(s) encoding the protein
Protein Source
|
Enterobacteria phage RB69
|
|
Defence system(s) inhibited by the protein
Defences
|
TA
|