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Mode of Action (MoA)
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None
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Evidence
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When expressed in E. coli, ArdB inhibited four major subfamilies of Type I restriction–modification systems (RM types IA–ID), allowing bacteriophages to infect cells that would otherwise restrict them. This shows that ArdB confers anti‑restriction protection inside living cells. However, purified ArdB did not block the restriction activity of a model enzyme (EcoKI) in test-tube assays (in vitro). That implies that ArdB doesn’t act by binding directly to the restriction enzyme’s active site.
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MoA Category
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unknown
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli type I RM
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Relevant publication(s)
DOI
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10.1093/nar/gkp1144
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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2WJ9
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Genome(s) encoding the protein
Protein Source
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Plasmid pKM101
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Defence system(s) inhibited by the protein
Defences
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RM
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