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Mode of Action (MoA)
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shares high similarity to ArdA (PDB ID: 2w82), which binds to the MTase of the type I RM complex and blocks it. AlphaFold 3 predicts a moderate-confidence complex between MTase (https://www.ncbi.nlm.nih.gov/protein/WP_010883983.1) and ArdU (ipTM = 0.76, pTM = 0.76).
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Evidence
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ArdU was identified as a putative antirestriction protein based on sequence similarity to known antirestriction proteins such as ArdA from Yersinia pestis plasmid pMT-1, and others from enterobacterial plasmids like pKM101 and ColIb-P9. It was predicted to be highly acidic (pI 4.25), a characteristic feature of known Ard proteins. To test its functional role, the ardU gene was removed from plasmid pI3, resulting in derivative pI5. Transformation assays in Deinococcus radiodurans R1 showed that deletion of ardU led to a 50- to 100-fold decrease in transformation frequency compared to wild-type pI3, indicating that ArdU enhances transformation efficiency. However, ArdU was not required for replication or stable maintenance of the plasmid. These results support a role for ArdU in overcoming host restriction barriers during plasmid uptake, consistent with antirestriction function. Binding it supported by computational structure modeling evidence (ECO_0006368).
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MoA Category
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binds and inhibits host defence system (putative)
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Deinococcus radiodurans RM systems
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Relevant publication(s)
DOI
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10.1128/AEM.66.9.3856-3867.2000
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Deinococcus radiopugnans plasmid pUE30
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Defence system(s) inhibited by the protein
Defences
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RM
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