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Mode of Action (MoA)
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disrupts the interaction between the inflammasome complex and caspase, preventing activation of the caspase.
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Evidence
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The evidence comes from a preprint (DOI: 10.1101/2023.05.28.542683), which later formed the basis of a peer-reviewed publication, though the specific experiments discussed here may were not included in the final paper. Co-expression of the CARD-like protein and the gasdermin system in cells led to increased phage sensitivity, implying inhibition of the defense. Infected cells expressing the CARD-like protein showed reduced gasdermin cleavage—a key step in gasdermin activation—supporting functional inhibition. Engineering the CARD-only gene into phage T4 under a native promoter resulted in T4 phages that were partially resistant to gasdermin-based immunity, confirming anti-defense activity in a different phage context.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Lysobacter Enzymogenes Gasdermins
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Relevant publication(s)
DOI
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10.1038/s41586-024-08498-3
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Acinetobacter phage 133
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Defence system(s) inhibited by the protein
Defences
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Gasdermin
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