{
  "id": "dcmp_hm",
  "name": "Deoxycytidylate hydroxymethylase",
  "moa": "converts 2\u2032-deoxycytidylate (or 2\u2032-deoxycytidine-5\u2032-monophosphate, dCMP) into 5-hydroxymethyl-dCMP (a step in DNA hypermodification).",
  "moa_category": "modifies phage molecules to avoid recognition",
  "evidence": "Crystal structure of T4 deoxycytidylate hydroxymethylase with dCMP shows the direct binding (ECO_0001034). Substitution of Asp179 to Asn drastically reduced activity on dCMP (~15,000-fold decrease in k_cat/K_M), but increased activity on dUMP, suggesting Asp179 is crucial for dCMP specificity and catalysis.",
  "defence_subtype": "Escherichia coli RM",
  "doi": "10.1093/emboj/18.5.1104",
  "multicomponent": "dnmp;bgt",
  "pdb": "1B5E_A",
  "structure_type": "PDB entry",
  "protein_source_name": "Enterobacteria phage T4",
  "protein_source_link": "https://www.ncbi.nlm.nih.gov/nuccore/NC_000866.4",
  "defences": [
    {
      "name": "RM",
      "link": "https://defensefinder.mdmlab.fr/wiki/defense-systems/rm"
    }
  ],
  "pfam_annotations": [
    {
      "name": "Thymidylat_synt",
      "accession": "PF00303.24",
      "length": 266,
      "evalue": 3.7e-18,
      "hmm_from": 53,
      "hmm_to": 219,
      "ali_from": 55,
      "ali_to": 226,
      "env_from": 18,
      "env_to": 238
    }
  ]
}