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Mode of Action (MoA)
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competes with the tail tube protein, DSR2's trigger, by binding DSR2 and inhibiting its NADase activity.
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Evidence
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Homologous recombination allowed transfer of the DSAD1 gene from DSR2-resistant phages (SPbeta or phi3T) to DSR2-sensitive SPR phages, rendering the hybrid SPR phages resistant to DSR2 defense. Co-expression of DSAD1 with DSR2 in B. subtilis inhibited DSR2-mediated protection against SPR infection (ECO_0000017), confirming DSAD1’s role as an anti-defense protein. Pulldown assays (ECO_0006249) demonstrated a direct physical interaction between DSAD1 and DSR2.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Bacillus subtilis 29R type II DSR
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Relevant publication(s)
DOI
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10.1038/s41564-022-01207-8
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Bacillus phages SP-beta and phi3T
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Defence system(s) inhibited by the protein
Defences
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DSR
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