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Mode of Action (MoA)
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AlphaFold 3 predicts a direct interaction between Avs3B (https://www.ncbi.nlm.nih.gov/protein/AZY97719.1) and Forsur-7 (ipTM = 0.72, pTM = 0.37).
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Evidence
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Forsur-7 was identified as a phage-encoded anti-defense factor through co-expression assays with SeAvs3 and its cognate trigger (the PhiV-1 large terminase subunit). In a genetic screen for phage early genes that rescue Avs-mediated toxicity, Forsur-7 partially restored E. coli growth when co-expressed with SeAvs3 and its activator. In vitro assays showed that Forsur-7 weakly inhibited SeAvs3 nuclease activity on double-stranded DNA, indicating it interferes with Avs3 effector function. Additionally, plaque assays demonstrated that Forsur-7 enhances phage propagation on Avs-containing E. coli, supporting its role as a functional Avs inhibitor. Binding it supported by computational structure modeling evidence (ECO_0006368).
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MoA Category
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binds and inhibits host defence system (putative)
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Salmonella enterica type III Avs
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Relevant publication(s)
DOI
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10.1126/science.abm4096
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Escherichia phage forsur
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Defence system(s) inhibited by the protein
Defences
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Avs
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