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Mode of Action (MoA)
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binds the GajAB complex, forming an octameric web around it that inhibits DNA recognition and cleavage.
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Evidence
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B. subtilis cells expressing Gabija show phage resistance, but co-expression of Gad1 with Gabija allowed phage SPĪ² to replicate, showing loss of defence. Mutation of Gad1 abolished this immune evasion. Electrophoretic mobility shift assays (ECO_0000096) and DNA cleavage assays demonstrated that GajAB normally cleaves sequence-specific phage DNA, but when Gad1 is present, GajAB is no longer able to bind DNA or perform cleavage. Pull-down (ECO_0006249) assays and cryo-EM structural analysis (ECO_0006181) show that Gad1 binds directly to the bacterial Gabija GajAB complex.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Bacillus cereus VD045 Gabija
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Relevant publication(s)
DOI
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10.1038/s41586-023-06855-2
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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8U7I_E
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Genome(s) encoding the protein
Protein Source
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Bacillus phage phi3T
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Defence system(s) inhibited by the protein
Defences
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Gabija
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