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Mode of Action (MoA)
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binds to the RecBCD complex and may bind to KiwaB. triggers retrons Se72 and Ec48, as well as Old nuclease.
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Evidence
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Purified Gam protein inhibits all catalytic activities of RecBC DNase (measured using using radiolabeled DNA substrates), including its ATPase (measured by tracking hydrolysis of [γ-³²P]ATP) and exonuclease functions. Two-hybrid (ECO_0000068) and pull-down (ECO_0006249) assay showed binding between KwaB and Gam.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli RecBCD and Kiwa
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Relevant publication(s)
DOI
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10.1073/pnas.70.8.2215,
10.1016/j.cell.2025.07.002
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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2UUZ
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Genome(s) encoding the protein
Protein Source
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Enterobacteria phage lambda
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Defence system(s) inhibited by the protein
Defences
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RecBCD,
Kiwa
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