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Mode of Action (MoA)
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binds and inhibits Dgt
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Evidence
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Gp1.2 was structurally characterized by NMR and shown to bind directly to Dgt, as confirmed by cryo-EM of the Dgt–Gp1.2 complex with or without bound (d)GTP (ECO_0006181). Biochemical assays showed Gp1.2 is a mixed-type inhibitor, reducing both Vmax and increasing KM, with an IC50 of 160 nM. GTP alone competitively inhibited Dgt (KI ~120 µM), but in combination with Gp1.2, inhibition was synergistic, reducing Gp1.2’s IC50 to 27 nM and GTP’s IC50 to 310 nM.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli dGTPase
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Relevant publication(s)
DOI
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10.1073/pnas.2123092119
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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7U66
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Genome(s) encoding the protein
Protein Source
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Escherichia phage T7
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Defence system(s) inhibited by the protein
Defences
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dGTPase
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