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Mode of Action (MoA)
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modifies adenine residues to N6-methyladenine.
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Evidence
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Hin1523 was cloned from the Haemophilus influenzae Rd strain. Like Hia5, it exhibited DNA methyltransferase activity in vitro. Using [³H]-AdoMet and λ DNA, incorporation of methyl groups was confirmed and was absent in the D194A mutant (ECO_0000315). Thin-layer chromatography (ECO_0000325) identified m6A as the modified base. REase protection assays showed resistance of plasmid DNA from Hin1523-expressing E. coli to multiple methylation-sensitive restriction enzymes, though the protection was less extensive than that seen with Hia5. HPLC revealed that Hin1523 methylated ~30% of adenines in λ DNA. Kinetic analysis showed preference for CA and TA dinucleotides, with GA methylated less efficiently. Poly(A) tracts remained unmethylated, suggesting similar minimal sequence specificity as Hia5 but with lower catalytic efficiency.
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MoA Category
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modifies phage molecules to avoid recognition
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Diverse set of REases from type I RM systems
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Relevant publication(s)
DOI
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10.1093/nar/gkr1039
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Prophage in Haemophilus influenzae
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Defence system(s) inhibited by the protein
Defences
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RM
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View homologs from eukaryotic dsDNA viruses
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