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Mode of Action (MoA)
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binds and inhibits the GmrS/GmrD complex (glucose-modified hydroxymethylcytosine restriction endonuclease).
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Evidence
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Ectopic expression (ECO_0000017) of IPI* in Escherichia coli rescues infection by T4 and other glucosyl-HMC-modified T-even phages from restriction by the GmrSD type IV endonuclease in plaque formation assays. Purified IPI* inhibits GmrSD nuclease activity in vitro (ECO_0000184). Site-directed mutagenesis of residue A40T abolishes IPI*-mediated inhibition of GmrSD (ECO_0001038).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli type IV RM
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Relevant publication(s)
DOI
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10.1016/j.jmb.2007.10.064
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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2JUB
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Genome(s) encoding the protein
Protein Source
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Enterobacteria phage T4
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Defence system(s) inhibited by the protein
Defences
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RM
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