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Mode of Action (MoA)
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phosphorylates Dnd, CRISPR-Cas, QatABCD, SIR2+HerA and DUF4297+HerA defence systems.
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Evidence
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Deletion of JSS1_004 (JSS1Δ004) completely abolished JSS1’s ability to evade Dnd-mediated immunity (ECO_0001038), resulting in up to six orders of magnitude reduction in efficiency of plating (EOP) on Dnd+ strains. Quantitative phosphoproteomic analysis identified 480 phosphorylation sites across 333 proteins during JSS1 infection, including multiple sites on the DndFGH complex. Purified JSS1_0041–262 (containing only the kinase domain) was shown to phosphorylate DndFGH in vitro. Phosphomimetic mutations (e.g., T22E, T23E, Y1510E) in DndF and DndH mimicked phosphorylation and abolished the antiphage activity of the DndFGH complex. To test activity against other defence systems (QatABCD, SIR2+HerA, DUF4297+HerA), the authors performed phage plaque assays in bacterial strains expressing these systems. JSS1Δ004 showed reduced EOP on these strains, indicating active defence. In contrast, wild-type JSS1 overcame these defences, demonstrating that JSS1_004 enables immune evasion beyond Dnd.
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MoA Category
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adds a post-translational modification and deactivates bacterial defence
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Salmonella enterica type ABCDEFGH Dnd, the type I-E CRISPR-Cas system, QatABCD, SIR2+HerA and DUF4297+HerA.
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Relevant publication(s)
DOI
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10.1038/s41564-024-01851-2
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Salmonella phage JSS1
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Defence system(s) inhibited by the protein
Defences
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Dnd,
CRISPR-Cas,
QatABCD,
SIR2+HerA,
DUF4297+HerA
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View homologs from eukaryotic dsDNA viruses
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