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Mode of Action (MoA)
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None
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Evidence
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Lar anti-restriction protein (Lar) was identified as a functional homolog of Ral through restriction alleviation and modification enhancement assays in E. coli strains expressing the Type IA R-M system EcoKI. Lar activity was confirmed by expression of lar from the Rac prophage in sbcA mutants (E.colu) and the hybrid phage λreverse. Cloning of lar into plasmids (e.g., pGK10, pGK15) enabled restriction alleviation as measured by increased phage titre of unmodified λ on restricting strains (ECO_0000017). Enhancement of modification was demonstrated by infecting cells expressing lar with unmodified phage and showing increased protection of progeny (ECO_0000017).
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MoA Category
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unknown
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli K-12 type 1 RM
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Relevant publication(s)
DOI
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10.1111/j.1365-2958.1995.tb02438.x
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Enterobacteria phage lambda
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Defence system(s) inhibited by the protein
Defences
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RM
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