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Mode of Action (MoA)
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mutated DNA polymerase capable of replicating DNA modified (ADP-ribosylated) by the host DarTG system.
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Evidence
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SECϕ18 phages were serially passaged on E. coli cells containing DarTG2 to select for escape mutants, mutations in mga47 were found in resistant phages. Overexpression of mga47 (wild-type and mutant) showed it does not trigger DarT2 activity. ELTA assays demonstrated that even in presence of mutant mga47, DarT2 was still active and ADP-ribosylated phage DNA, suggesting the polymerase mutations allowed phage to tolerate or bypass DarT2’s modification (ECO_0005801).
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MoA Category
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uncategorised
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli type IV TA system DarTG
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Relevant publication(s)
DOI
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10.1038/s41564-022-01153-5
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Phage SECϕ18
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Defence system(s) inhibited by the protein
Defences
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TA
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