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Mode of Action (MoA)
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Recognises the sequence 5'-(C or G)-A-(C or G)-N-(C or T)-3' and performs methylcarbamoylation of adenine, preventing recognition of phage DNA by type I (EcoKI and EcoBI) restriction nucleases.
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Evidence
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Ectopic expression of the gene renders phages resistant to numerous restriction enzymes (ECO_0000017). The modification (methylcarbamoylation) was identified using liquid chromatography–electrospray ionisation mass spectrometry (ECO_0001582).
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MoA Category
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modifies phage molecules to avoid recognition
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli type I and II RM
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Relevant publication(s)
DOI
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10.1016/0042-6822(76)90232-4,
10.1016/0378-1119(85)90108-8,
10.1093/nar/gkaa319
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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8BV8_A
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Genome(s) encoding the protein
Protein Source
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Escherichia phage Mu
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Defence system(s) inhibited by the protein
Defences
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RM
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