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Mode of Action (MoA)
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modifies adenine residues to N6-methyladenine.
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Evidence
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Nma1821 was cloned from Neisseria meningitidis type A strain Z2491. It demonstrated strong DNA methyltransferase activity in vitro, similar to Hia5. Incorporation of [³H]-methyl groups into DNA was confirmed and lost in the D191A mutant (ECO_0000315). Thin-layer chromatography (ECO_0000325) showed the methylated base to be m6A. Plasmid DNA from E. coli expressing Nma1821 was resistant to most of the same methylation-sensitive REases tested for Hia5, indicating extensive adenine methylation. While HPLC data specific to Nma1821 was not shown, its restriction protection profile was highly similar to that of Hia5, implying similarly widespread, non-specific adenine methylation. This positions Nma1821 as a highly active, non-specific DNA adenine methyltransferase
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MoA Category
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modifies phage molecules to avoid recognition
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Diverse set of REases from type I RM systems
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Relevant publication(s)
DOI
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10.1093/nar/gkr1039
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Prophage in Haemophilus influenzae
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Defence system(s) inhibited by the protein
Defences
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RM
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View homologs from eukaryotic dsDNA viruses
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