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Mode of Action (MoA)
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directly binds the ATPase BrxC, disrupting its dimerisation
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Evidence
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Deletion (ECO_0001038) of orbA abolished plaque formation and phage genome replication in Vibrio cholerae strains expressing the BREX system from ICEVchInd5. This restriction was relieved either by restoring orbA or deleting the brxC component of BREX, confirming OrbA’s role in counter-defense. Co-immunoprecipitation (ECO_0005644) experiments revealed that OrbA binds directly to BrxC, the ATPase component of BREX. A bacterial two-hybrid assay (ECO_0005805) confirmed this interaction occurs specifically with the Ind5 BrxC variant, which OrbA inhibits, but not with BrxC from BREX systems that resist OrbA. Furthermore, OrbA expression disrupted BrxC multimerization in vivo, a process dependent on intact Walker A and B ATPase motifs, suggesting OrbA inhibits BREX by interfering with BrxC oligomerization and function.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Vibrio cholerae type I BREX
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Relevant publication(s)
DOI
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10.1126/science.abg2166,
10.1128/jb.00206-24
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Vibrio phage ICP1
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Defence system(s) inhibited by the protein
Defences
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BREX
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