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Mode of Action (MoA)
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predicted to act as a nick-sensing, ATP-dependent DNA ligase (ipTM = 0.95, pTM = 0.95) that reverses the effect of effector nucleases.
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Evidence
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ORF55 was identified via a knockout screen where its deletion led to >100-fold reduction in infectivity against E. coli expressing AVAST type III, accompanied by small plaque formation. Complementation of the ΔORF55 mutant with a plasmid-borne ORF55 restored infectivity and plaque size, confirming its anti-AVAST function. Binding nicked DNA and ATP is supported by computational structure modeling evidence (ECO_0006368).
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MoA Category
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synthesises and restores essential molecules depleted by bacterial defence (putative)
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli type III Avs
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Relevant publication(s)
DOI
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10.1101/2024.04.14.589459
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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DruSM1 phage
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Defence system(s) inhibited by the protein
Defences
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Avs
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View homologs from eukaryotic dsDNA viruses
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